REPRODUCTION IN PLANTS AND ANIMALS

Introduction

• The process by which mature individuals produce offspring is called reproduction.
• Reproduction is a characteristic of all living organisms and prevents extinction of a species.
• There are two types of reproduction: sexual and asexual reproduction.
• Sexual reproduction involves the fusion of male and female gametes to form a zygote.
• Asexual reproduction does not involve gametes.

Cell Division

• Cell division starts with division of nucleus.
• In the nucleus are a number of thread-like structures called chromosomes, which occur in pairs known as homologous chromosomes.
• Each chromosome contains-genes that determine the characteristics of an organism.
• The cells in each organism contains a specific number of chromosomes.

There are two types of cell division:

Mitosis –

• This takes place in all body cells of an organism to bring about increase in number of cells, resulting in growth and repair.
• The number of chromosomes in daughter cells remain the same as that in the mother cell.

Meiosis –

• This type of cell division takes place in reproductive organs (gonads) to produce gametes.
• The number of chromosomes in the gamete is half that in the mother cell.

Mitosis

• Mitosis is divided into four main stages.
• Prophase, Metaphase, Anaphase and Telophase.
• These stages of cell division occur in a smooth and continuous pattern.

 

Interphase

• The term interphase is used to describe the state of the nucleus when the cell is just about to divide.
• During this time the following take place:
• Replication of genetic material so that daughter cells will have the same number of chromosomes as the parent cell.
• Division of cell organelles such as mitochondria, ribosomes and centrioles.
• Energy for cell division is synthesised and stored in form of Adenosine Triphosphate (ATP) to drive the cell through the entire process.
• During. interphase, the following observations can be made:
• Chromosomes are seen as long, thin, coiled thread-like structures.
• Nuclear membrane and nucleolus are intact.

Prophase

• The chromosomes shorten and thicken.
• Each chromosome is seen to consist of a pair of chromatids joined at a point called centromere.
• Centrioles (in animal cells) separate and move to opposite poles of the cell.
• The centre of the nucleus is referred to as the equator.
• Spindle fibres begin to form, and connect the centriole pairs to the opposite poles.
• The nucleolus and nuclear membrane disintegrate and disappear.

Metaphase

• Spindle fibres lengthen.
• In animal cells they attach to the centrioles at both poles.
• Each chromosome moves to the equatorial plane and is attached to the spindle fibres by the centromeres.
• Chromatids begin to separate at the centromere.

Anaphase

• Chromatids separate and migrate to the opposite poles due to the shortening of spindle fibres .
• Chromatids becomes a chromosome.
• In animal cell, the cell membrane starts to constrict. 

Telophase

• The cell divides into two.
• In animal cells it occurs through cleavage of cell membrane.
• In plants cells, it is due to deposition of cellulose along the equator of the cell.(Cell plate formation).
• A nuclear membrane forms around each set of chromosome.
• Chromosomes later become less distinct.

Significance of Mitosis

• It brings about the growth of an organism:
• It brings about asexual reproduction.
• Ensures that the chromosome number is retained.
• Ensures that the chromosomal constitution of the offspring is the same as the parents.

Meiosis

• Meiosis involves two divisions of the parental cell resulting into four daughter cells.
• The mother cell has the diploid number of chromosomes.
• The four cells (gametes) have half the number of chromosomes (haploid) that the mother cell had.
• In the first meiotic division there is a reduction in the chromosome number because homologous chromosomes and not chromatids separate.
• Each division has four stages Prophase, Metaphase, Anaphase and Telophase. 

Interphase

• As in mitosis the cell prepares for division.
• This involves replication of chromosomes, organelles and build up of energy to be used during the meiotic division.

First Meiotic division

Prophase I

• Homologous chromosomes lie side by side in the process of synapsis forming pairs called bivalents.
• Chromosomes shorten and thicken hence become more visible.
• Chromosomes may become coiled around each other and the chromatids may remain in contact at points called chiasmata (singular chiasma).
• Chromatids cross-over at the chiasmata exchanging chromatid portions. Important genetic changes usually result.

Metaphase I

• Spindle fibres are fully formed and attached to the centromeres.
• The bivalents move to the equator of the spindles.

Anaphase I

• Homologous chromosomes separate and migrate to opposite poles.
• This is brought about by shortening of spindle fibres hence pulling the chromosomes.
• The number of chromosomes at each pole is half the number in the mother cell.

Telophase I

• Cytoplasm divides to separate the two daughter cells.

Second Meiotic Division

• Usually the two daughter cells go into a short resting stage (interphase)
• but sometimes the chromosomes remain condensed and the daughter cells go straight into metaphase of second meiotic division.
• The second meiotic division takes place just like mitosis.

Prophase II

• Each chromosome is seen as a pair of chromatids.

Metaphase II

• Spindle forms and are attached to the chromatids at the centromeres.
• Chromatids move to the equator.

Anaphase II

• Sister chromatids separate from each other
• Then move to opposite poles, pulled by the shortening of the spindle fibres.

Telophase II

• The spindle apparatus disappears.
• The nucleolus reappears and nuclear membrane is formed around each set of chromatids.
• The chromatids become chromosomes.
• Cytoplasm divides and four daughter cells are formed.
• Each has a haploid number of chromosomes.

Significance of Meiosis

• Meiosis brings about formation of gametes that contain half the number of chromosomes as the parent cells.
• It helps to restore the diploid chromosomal constitution in a species at fertilisation.
• It brings about new gene combinations that lead to genetic variation in the offsprings.

Asexual Reproduction

• Asexual reproduction is the formation of offspring from a single parent.
• The offspring are identical to the parent.

   Types of asexual reproduction.

• Binary fission in amoeba.
• Spore formation in Rhizopus.
• Budding in yeast.

Binary fission

• This involves the division of the parent organism into two daughter cells.
• The nucleus first divides into two and then the cytoplasm separates into two portions
• Binary fission also occurs in bacteria, Paramecium, Trypanosoma and Euglena.

Spore formation in Rhizopus

• Rhizopus is a saprophytic fungus which grows on various substrate such as bread, rotting fruits or other decaying organic matter.
• The vegetative body is called mycelium which has many branched threads called hyphae.
• Horizontal hyphae are called stolons.
• Vertical hyphae are called sporangiophore.
•  The tips of sporangiophore become swollen to form sporangia, the spore bearing structure.
• Each sporangium contains many spores.
• As it matures and ripens, it turns black in colour.
• When fully mature the sporangium wall burst and release spores which are dispersed by wind or insects.
• When spores land on moist substratum, they germinate and grow into a new Rhizopus and start another generation.

 

Spore formation in ferns

• The fern plant is called a sporophyte.
• On the lower side of the mature leaves are sari (Singular: sorus) which bear spores.

 

Budding in Yeast

• Budding involves the formation of a protrusion called a bud from the body of the organism.
• The bud separates from the parent cell, in yeast budding goes on so fast and the first bud starts to form another bud before the separation.
• A short chain or mass of cells is formed.

 

Sexual Reproduction in Plants

• In flowering plants, the flower is the reproductive organ which is a specialised shoot consisting of a modified stem and leaves.
• The stem-like part is the pedicel and receptacle, while modified leaves form corolla and calyx.

 

Structure of a flower

• A typical flower consists of the following parts:

Calyx –

• made up of sepals.
• They enclose and protect the flower when it is in a bud. Some flowers have an outer whorl made of sepal-like structures called epicalyx.

Corolla –

• consists of petals. The petals are brightly coloured in insect – pollinated flowers.

Androecium –

• Is the male part of the flower. It consists of stamens.
• Each stamen consists of a filament whose end has an anther.
• Inside the anther are pollen sacs which contain pollen grains.

Gynoecium (pistil) –

• Is the female part of the flower.
• It consists of one or more carpels.
• Each carpel consists of an ovary, a sty le and a stigma.
• The ovary contains ovules which become seeds after fertilisation.

• A monocarpous pistil has one carpel e.g.  beans.
• A polycarpous pistil has many carpels.
• If the carpes are free, it is called apocarpous as in rose and Bryophyllum,
• In carpels that are fused it is called syncarpous as in Hibiscus.
• A complete flower has all the four floral parts.
• A regular flower can be divided into two halves by any vertical section passing through the centre. e.g. morning glory.
• Irregular flower can be divided into two halves in only one plane e.g. crotalaria.

 

Pollination

• This is the transfer of pollen grains from the anther to the stigma.

Types of pollination

• Self pollination is the transfer of pollen grains from the anther of one flower to the stigma of the same flower.
• Cross-pollination is the transfer of pollen grains from the anther of one flower to the stigma of a different flower, of the same species.

 Agents of pollination

• Agents of pollination include wind, insects, birds and
• Insect pollinators include bees, butterflies and mosquitoes.

 Mechanisms that hinder self-pollination

• Stamens ripen early and release their pollen grains before the stigma, mature. This is called protandry e.g. in sunflower.
• The stigma matures earlier and dries before the anthers release the pollen grains.
• This is called protogyny and is common in grasses.
• Self sterility or incompatibility
• Pollen grains are sterile to the stigma of the same flower, e.g. in maize flower.
• Shorter stamens than pistils.

Fertilisation in Plants

• The pollen grain contains the generative nucleus and a tube nucleus.
• When the pollen grain lands on the stigma, it absorbs nutrient and germinates forming a pollen tube.
• This pollen tube grows through the style pushing its way between the cells.
• It gets nourishment from these cells.
• The tube nucleus occupies the position at the tip of the growing pollen tube.
• The generative nucleus follows behind the tube nucleus, and divides to form two male gamete nuclei.
• The pollen tube enters the ovule through the micropyle.
• When the pollen tube penetrates the ovule disintegrates and the pollen tube bursts open leaving a clear way for the male nuclei.
• One male nucleus fuses with the egg cell nucleus to form a diploid zygote which develops into an embryo.
• The other male gamete nucleus fuses with the polar nucleus to form a triploid nucleus which forms the primary endosperm.
• This is called double fertilisation.

 After fertilisation the following changes take place in a flower:

• The integuments develops into seed coat (testa).
• The zygote develops into an embryo.
• The triploid nucleus develops into an endosperm.
• The ovules become seeds.
•  The ovary develops into a fruit.
• The ovary wall develops into pericarp.
• The style, dries up and falls off leaving a scar.
• The corolla, calyx and stamens dry up and fall off.
•  In some the calyx persists.

Fruit formation

• Fruit development without fertilisation is called parthenocarpy
• g. as in pineapples and bananas.
• Such fruits do not have seeds.

 

Classification of fruits

• False fruits develops from other parts such as calyx, corolla and receptacle,
• e.g. apple and pineapple which develops from an inflorescence.
• True fruits develop from the ovary, e.g. bean fruit (pod).
• True fruits can be divided into fleshy or succulent fruits e.g. berries and drupes and dry fruits.
• The dry ones can be divided into Dehiscent which split open to release seeds and indehiscent which do not open.

Placentation

• This is the arrangement of the ovules in an ovary.

Marginal placentation:

• The placenta appears as one ridge on the ovary wall e.g. bean.

Parietal placentation:

• The placenta is on the ridges on ovary wall.
• Ovules are in them e.g. pawpaw.

Axile placentation:

• The placenta is in the centre.
• Ovary is divided into a number of loculi. e.g. orange.

Basal placentation.

• The placenta is formed at the base of the ovary e.g. sunflower.

Free Central placentation.

• Placenta is in the centre of the ovary.
• There are no loculi e.g. in primrose.

 

Methods of fruit and seed dispersal

Animal dispersal

• Fleshy fruits are eaten by animals.
• Animals are attracted to the fruits by the bright colour, scent or the fact that it is edible.
• The seeds pass through the digestive tract undamaged and are passed out with faeces. E.g. tomatoes and guavas.
• Such seeds have hard, resistant seed coats.
• Others have fruits with hooks or spines that stick on animal fur or on clothes.
• Later the seeds are brushed of or fall off on their own e.g. Bidens pilosa (Black jack).

 

Wind dispersal

• Fruits and seeds are small and light in order to be carried by air currents.
• A fruit that is a capsule e.g. tobacco split or has pores at the top e.g. Mexican poppy.
• The capsule is attached to along stalk when swayed by wind the seeds are released and scattered.
• Some seeds have hairy or feather-like structures which increase their surface area so that they can be blown off by the wind e.g. Sonchus.
• Others have wing-like structures e.g. Jacaranda and Nandi Flame.
• These extensions increase the surface area of fruits and seeds such that they are carried by the wind.

 

Water dispersal

• Fruits like coconut have fibrous mescocarp which is spongy to trap air, the trapped air make the fruit light and buoyant to float on water.
• Plants like water lily produce seeds whose seed coats trap air bubbles.
• The air bubbles make the seeds float on water and are carried away.
• The pericarp and seed coat are waterproof.

 

Self dispersal (explosive) Mechanism

• This is seen in pods like bean and pea.
• Pressure inside the pod forces it to open along lines of weakness throwing seeds away from parent plant.

Reproduction in Animals

• Sexual reproduction involves the fusion of gametes.
• In animals two individuals are involved, a male and a female.
• Special organs known as gonads produce gametes.
• In males testes produce sperms while in females ovaries produce ova.
• The fusion of male gamete and female gamete to form a zygote is called fertilisation.

There are two types of fertilisation. External and internal.

External fertillsation

• Example in amphibians takes place in water.
• The male mounts the female and shed sperms on the eggs as they are laid.
• Eggs are covered by slippery jelly-like substance which provides protection.
• Many eggs are released to increase the chances of survival.

 Internal fertilisation

• This occurs in reptiles, birds and mammals.
• Fertilisation occurs within the body of the female.
• Fewer eggs are produced because there are higher chances of fertilisation since sperms are released into the female body.

Reproduction in Humans  

Structure of female reproduction system  

The female reproduction system consist of the following:

Ovaries

• Are two oval cream coloured structures found in lower abdomen below the kidneys.

Oviducts.

• They produce the ova.
• Are tubes which conduct the ova produced by the ovaries to the uterus.
• Fertilisation occurs in the upper part of the oviduct.

Uterus

• The uterus is a hollow muscular organ found in the lower abdomen.

• The embryo develops inside the uterus.
• The inner lining endometrium supplies nutrients to embryo.
• The embryo is implanted into the inner uterine wall- the endometrium which nourishes the embryo.
• The thick muscles of the uterus assist in parturition.

Cervix

• Has a ring of muscles that separates the uterus from the vagina.
• It forms the opening to the uterus

Vagina

• Is a tube that opens to the outside and it acts as the copulatory and birth canal through the vulva.

Structure of male reproductive system

 The male reproductive system consists of the following:

Testis:

• Each testis is a mass of numerous coiled tubes called semniferous tubules.
• Each is enclosed within a scrotal sac that suspends them between the thighs.
• This ensures that sperms are maintained at a temperature lower than that of the main body.

Seminiferous tubules

• The lining of seminiferous tubules consists of actively dividing cells which give rise to sperms.
• Between the seminiferous tubules are interstitial cells which produce the male hormones called androgens e.g. testosterone.
• The seminiferous tubules unite to form the epididymis, which is a coiled tube where sperms are stored temporarily .
• Vas deferens (sperm duct) is the tube through which sperms are carried from testis to urethra.
• Seminal vesicle produces an alkaline secretion which nourishes the spermatozoa.

Prostate gland

• Produces an alkaline secretion to neutralise vaginal fluids.

Cowpers’ gland

• Secretes an alkaline fluid.
• All these fluids together with spermatozoa form semen.

Urethra

• Is a long tube through which the semen is conducted during copulation.
• It also removes urine from the bladder.

Penis

• Is an intro-mittent organ which is inserted into the vagina during copulation .

Fertilisation in Animals

• Fertilisation is preceded by copulation in which the erect penis is inserted into the vagina.
• This leads to ejaculation of semen.
•  The sperms swim through the female’s genital tract to the upper part of the oviduct.
•  The  head of the sperm penetrates the egg after the  acrosome_ releases lytic enzymes t dissolve the egg membrane.
• The tail is left  behind.
•  Sperm nucleus fuses with that of the ovum and a zygote is formed.
• A fertilisation membrane forms around the zygote which prevents other sperms from penetrating the zygote.

 Implantation:

• After fertilisation the zygote begins to divide mitoticaly as it moves towards the uterus.
•  It becomes embedded in the wall of the uterus a process called implantation.
• By this time the zygote is a hollow ball of cells called blastocyst or embryo.
• In the uterus the embryo develops villi which project into uterus for nourishment later the villi and endometrium develop into placenta.

Embryonic membranes

• Embryonic membranes develop around the embryo.
• The outermost membrane is the chorion which forms the finger-like projections (chorionic villi) which supply nutrients to the embryo.
• The amnion surrounds the embryo forming a fluid filled cavity within which the embryo lies.
• Amniotic cavity is filled with amniotic fluid.
• This fluid acts as a shock absorber and  protects the foetus against mechanical injury.
• It also regutates  temperature.
• The chorionic villi, allantois together with the endometrium from the placenta.
• The embryo is attached to the placenta by a tube called umbilical cord which has umbilical vein and artery.
• The maternal blood in the placenta flows in the spaces lacuna and surrounds capillaries from umbilical vein and artery.
• The umbilical cord increase in length as the embryo develops.

 

Role of placenta

    Protection

• Maternal blood and foetal blood do not mix.
• This ensures that the pathogens and toxins from maternal blood do not reach the foetus.
• The placenta allows maternal antibodies to pass into the foetus, providing the foetus with immunity.

     Nutrition

• The placenta facilitates the transfer of nutrients from maternal blood to foetus.

     Excretion

• Placenta facilitates the removal of nitrogenous wastes from the foetus’ blood to maternal blood.

      Gaseous exchange

• Oxygen from the maternal blood diffuses into the foetal blood while carbon (IV) oxide from foetal blood diffuse into maternal blood.

      Production of hormones

• Placenta produces progesterone and oestrogen.

Gestation period

• The period between conception and birth is called gestation.
• In humans gestation takes nine months (40 weeks).
• The embryo differentiates into tissues and organs during this period.

Week 1 to 3:

• Zygote divides to form blastocyst.
•  Implantation takes place.
• The three germ layers form endoderm, mesoderm and ectoderm.
• Nervous system starts to form.

Week 4 to 7:

• Development of circulating and digestive systems.
• Further development of nervous system, formation of sensory organs,
• All major internal organs are developed.
• At week 5, heartbeat starts .

Week 8 to 24:

• All organs well developed including sex organs.
• Hair, finger and toe nails grow.
• Foetus move and eyelids open.

Week 25- 30:

• The fully developed foetus responds to touch and noises and moves vigorously.
• The head turns and faces downwards ready for birth.

Week 31-40:

• Foetus increases in size.
• Birth occurs.

Secondary Sexual Characteristics

Male

• Testerone is the main androgen that stimulates the development of secondary sexual characteristics.
• Broadening of the shoulders.
• Deepening of the voice due to enlargement of larynx.
• Hair at the pubic area, armpit and chin regions.
• Penis and testis enlarge and produce sperms.
• Body becomes more masculine.

Female

• Enlargement of mammary glands.
• Hair grows around pubic and armpit regions.
• Widening of the hips.
• Ovaries mature and start producing ova.
• Menstruation starts.
• Oestrogen triggers the onset of secondary sexual characteristics.

Menstrual Cycle

• This is characterized by discharge of blood and tissue debris (menses) from the uterus every 28 days.
• This is due to the breakdown of the endometrium which occurs when the level of progesterone falls and the girl starts to menstruate.
• The follicle stimulating hormone (FSH) causes the Graafian follicle to develop and also stimulate the ovary to release oestrogen.
• Oestrogen hormone triggers the onset of secondary sexual characteristics.
• Luteinising hormone (L.H) causes the mature ovum to be released from the Graafian follicle – a process called ovulation.
• After ovulation progesterone hormone is produced.
• After menstruation, the anterior lobe of the pituitary gland starts secreting the follicle stimulating hormone (FS.H) which causes the Graafian follicle to develop in the ovary.
• It also stimulates the ovary tissues to secrete oestrogen.
• Oestrogen brings about the repair and healing of the inner lining of the uterus (endometrium) which had been destroyed during menstruation.
• Oestrogen level stimulates the pituitary gland to produce (Luteinising Hormone (L.H).
• This hormone makes the mature Graafian follicle to release the ovum into the funnel of oviduct, a process called ovulation.
• After releasing the ovum, the Graafian follicle changes into a yellow body called corpus luteum.
• The luteinising hormone stimulates the corpus luteum to secrete a hormone called progesterone which stimulates the thickening and vascularisation of endometrium.
• This prepares the uterine wall for implantation of the blastocyst.
• If fertilisation takes place, the level of progesterone increases and thus inhibits FSH from stimulating the maturation of another Graafian follicle.
• If fertilisation does not occur, the corpus luteum disintegrates and the level of progesterone goes down.
• The endometrium, sloughs off and menstruation occurs.

 

Advantages of Reproduction Asexual

• Good qualities from parents are retained in the offspring without variation.
• New individuals produced asexually mature faster.
• Process does not depend on external factors which may fail such as pollination.
• New individuals obtain nourishment from parent and so are able to survive temporarily under unsuitable conditions.
• No indiscriminate spreading of individuals which can result in wastage of offspring.
• Takes a shorter time and leads to rapid colonization.

 

Disadvantages of asexual reproduction

• New offspring may carry undesirable qualities from parents.
• Offspring may be unable to withstand changing environmental conditions.
• Faster maturity can cause overcrowding and stiff competition.
• Reduced strength and vigour of successive generations.

 

Advantages of sexual reproduction

• Leads to variations.
• Variations which are desirable often show hybrid vigour.
• High adaptability of individuals to changing environmental conditions.
• Variations provide a basis for evolutionary changes.

Disadvantages of sexual reproduction

• Fusion is difficult if two individuals are isolated.
• Some variations may have undesirable qualities.
• Population growth is slow.

Practical Activities

Examining the stages of mitosis

• About 2 mm of a root tip of onion bulb is cut off and placed on a microscope slide.
• A stain e.g. aceto-orcein is added and the root tip macerated using a scapel.
• A cover slip is added and observations made.
• Different stages of mitosis can be observed.

Examining the stages of meiosis

• An unopened bud of Tradescantia is obtained
• The anther is removed and placed on a microscope slide.
• A few drops of hydrochloric acid and acetic-orcein stain are added.
• A cover slip is placed on the anther.
• Pressing the cover slip gives a thin squash, which is observed under the microscope.
• Different stages of meiosis are observed.

To observe the structure of Rhizopus

• Rhizopus grow on moist bread left under suitable temperature
• A piece of moist bread is placed on a petri­-dish or enclosed in a plastic bag and observe daily for four days.
• Under a low power microscope the sporangia and stolons can be observed.

To examine spores on sori of ferns

• Obtain the fern plant.
• Detach a frond from the plant and observe the under-side using a hand lens to see the raised brown patches – the sori.
• Open up the sorus to observe the sporangia.

Examine insect and wind pollinated flowers

• Obtain insect pollinated flowers e.g. crotalaria, hibiscus/Ipomea, Solanum, incunum.
• Note the scent, colour and nectar guides.
• A description of the calyx, corolla, androecium and gynoecium is made.
• Obtain a wmd pollinated flower e.g,’ maize, star-grass, sugar-cane, Kikuyu grass.
• Observe the glumes, spikes and spikelet.
• Examine a single floret, and identify the androecium and gynoecium.

 

Classifying fruits

• Obtain different fruits – oranges, mangoes, maize, castor oil, bean pod, black jack .
• Observe the fruits, classify them into succulent, dry-dehiscent or indehiscent.

Dissection of Fruits

• Obtain an orange and a mango fruit.
• Make a transverse section.
• Observe the cut surface and draw and label the parts.
• Note that the fruit is differentiated into epicarp, mesocarp and endocarp.
• Obtain a pod of a legume.
• Open up the pod and observe the exposed surface.
• Draw and label the parts.
• Note that the fruit wall is not differentiated.

Dispersal of fruits and seeds

• Obtain animal dispersal fruits, like oranges, tomatoes, black jack, sodom apple.
• Identify the way by which each is adapted to dispersal by animals.
• Obtain wind dispersed fruit/seed

e.g. Nandi flame, Jacaranda Sonchus, cotton seed, Tecoma.